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gapdh 14c10 rabbit mab  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc gapdh 14c10 rabbit mab
    Gapdh 14c10 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 12230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh+14c10+rabbit+mab/GAPDH+Rabbit+mAb/pm41871791-56-114-118
    Average 99 stars, based on 12230 article reviews
    gapdh 14c10 rabbit mab - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Obesity promotes urinary tract infection by disrupting bladder focal adhesion kinase signaling
    Article Snippet: GAPDH (14C10) Rabbit mAb , Cell Signaling Technology , Cat#2118; RRID: AB_561053.

    MTT Assay:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    RNA Extraction:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    Isolation:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    Membrane:

    Article Title: Spatiotemporal dynamics of ecto-5′-nucleotidase (CD73) in mouse retina under physiological conditions
    Article Snippet: .. Proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, IPHV00010), blocked and immunoblotted with the following primary antibodies: NT5E/CD73 (D7F9A) rabbit mAb (1:1000, Cell Signaling, 13160; RRID: AB_2716625) and GAPDH (14C10) rabbit mAb (1:1000, Cell Signaling, 2118; RRID: AB_561053). .. Membranes were then incubated with anti-rabbit HRP-conjugated secondary antibodies (1:1000, Bio-Rad, 170-6515; RRID: AB_11125142) for 1 h at room temperature.

    Clinical Proteomics:

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera.
    Article Snippet: For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1: 10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1~2 hours. .. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al.66), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al.67) Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), PeroxidaseAffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115- AR TI CL E IN P RE SS ARTICLE IN PRESS 035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211). .. IP of cellular proteins: IRE1.LD antibodies were conjugated to agarose beads using the AminoLink® Plus Immobilization Kit (ThermoFisher, #44894).

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera
    Article Snippet: Signal was detected using appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies at 1:10,000 in 5% milk at room temperature for 1–2 h. Bands were visualized by the Azure 600 western blot imaging system (Azure Biosystems). .. For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1:10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1–2 h. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al. ), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al. ). .. Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115-035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211).

    In Vitro:

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera
    Article Snippet: Signal was detected using appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies at 1:10,000 in 5% milk at room temperature for 1–2 h. Bands were visualized by the Azure 600 western blot imaging system (Azure Biosystems). .. For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1:10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1–2 h. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al. ), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al. ). .. Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115-035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211).

    Ubiquitin Proteomics:

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera
    Article Snippet: Signal was detected using appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies at 1:10,000 in 5% milk at room temperature for 1–2 h. Bands were visualized by the Azure 600 western blot imaging system (Azure Biosystems). .. For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1:10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1–2 h. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al. ), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al. ). .. Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115-035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211).

    Blocking Assay:

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera
    Article Snippet: Signal was detected using appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies at 1:10,000 in 5% milk at room temperature for 1–2 h. Bands were visualized by the Azure 600 western blot imaging system (Azure Biosystems). .. For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1:10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1–2 h. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al. ), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al. ). .. Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115-035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211).



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